Composite

Part:BBa_K2680276

Designed by: Ethan M Jones   Group: iGEM18_William_and_Mary   (2018-10-06)


3G mf-Lon

mf-Lon flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page]. From BBa_K2333011:

"This part codes for the AAA+ Lon Protease from Mesoplasma florum bacteria (called mf-Lon for short). While it is evolutionarily related, and mechanistically similar to the E. coli Lon protease, mf-Lon is unable to recognize E. coli degradation tags (commonly known as ssRA tags), and vice versa. The orthogonal nature of this protease was notably used by Collins et al. 2014 ("Tunable Protein Degradation in Bacteria"), where a series of mf-Lon associated protein degradation tags were designed via mutagenesis to have distinct levels of affinity for the protease and therefore distinct associated degradation rates. These tags are available on the registry in the range BBa_K2333001-Bba_K2333006."

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1882
    Illegal AgeI site found at 1966
    Illegal AgeI site found at 2172
    Illegal AgeI site found at 2197
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41
    Illegal BsaI.rc site found at 2421


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Categories
Parameters
None